Cherry Chen | Senior Product Manager

DNA Separation for Paternity and Personal Identification

How is it possible to identify a person from a single hair, a cigarette butt or traces of blood? As a rule, biochemical methods must be used to extract DNA from hair, cigarette butts or traces of blood. Before DNA quantification, analysis of the sex and STR polymorphism of human DNA and sequencing of human mitochondrial DNA can be performed, the DNA must be isolated and purified. What is the best method to better isolate and purify DNA from trace amounts and complex sample matrices such as hair to obtain the most accurate results?

DNA ISOLATION AND PURIFICATION

First, the hair is cut into 3-5 mm long pieces, rinsed sequentially with ethanol and pure water, sun-dried, DTT, SDS and proteinase K and proteolysis buffer are added and digested at 37 °C until complete dissolution. The target DNA is then extracted again with organic solvents, mixed with NaCl and anhydrous ethanol and centrifuged at high speed to obtain the target DNA precipitate.

THE ROLE OF THE IKA G-L CENTRIFUGE

The IKA G-L centrifuge is compact and powerful, making it the perfect choice for isolating and purifying your target DNA.

Compact
IKA G-L has a very small footprint and requires only a small space of approx. 200 x 280 mm on your lab bench.

Powerful
With a maximum speed of up to 15,700 rpm, the IKA G-L is the most powerful device in its class. Substances can be easily separated from the sample matrix. The capacity is 12 x 1.5/2.0 ml.

Safe
IKA G-L is equipped with an imbalance sensor. If the Falcon tubes are not positioned correctly, the device cannot be started and issues a warning. This prevents the motor from jamming or even starting.
Falcon tubes must be inserted diagonally.

Easy to operate
With the IKA G-L you no longer have to carry out complicated calculations. At the touch of a button, you can choose between the units RCF (relative centrifugal force) or RPM (revolutions per minute).

RPM indicates the speed at which the rotor turns. RCF is the term for the acceleration force acting on a sample in a centrifuge.